normal human fetal osteoblast cell line  (ATCC)

Journal: Biomedicines

Article Title: CAPE Derivatives as Potent Agents for Induction of Osteogenic Differentiation in DPSCs and Biomaterial Development

doi: 10.3390/biomedicines13123039

Figure Lengend Snippet: Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 osteoblasts. ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).

Article Snippet: Normal human fetal osteoblast cell line (hFOB 1.19, ATCC CRL-11372) was cultured in a 1:1 mixture of Dulbecco’s Modified Eagle’s Medium and Ham’s F12 Medium, supplemented with 2.5 mM L-glutamine, 0.3 mg/mL G418 (Sigma-Aldrich Chemicals, Warsaw, Poland), and 10% FBS (Pan-Biotech GmbH, Aidenbach, Bavaria, Germany) and maintained at 34 °C (5% CO 2 in air atmosphere).

Techniques: MTT Assay, Cell Culture, Negative Control, Control, Laser-Scanning Microscopy, Staining, Fluorescence

Journal: Frontiers in Immunology

Article Title: A novel hypoxia- and lactate metabolism-related prognostic signature to characterize the immune landscape and predict immunotherapy response in osteosarcoma

doi: 10.3389/fimmu.2024.1467052

Figure Lengend Snippet: Expression and biological functions on growth of SFXN4 and SQOR in osteosarcoma. (A) SFXN4 and SQOR expression in para-cancerous and osteosarcoma tissues by IHC. (B, C) The expression of SFXN4 and SQOR at RNA and protein levels in normal osteoblast cell and osteosarcoma cell lines through qRT-PCR and western blotting assays. (D, E) Effect of knocking down SFXN4 and SQOR at RNA and protein levels in osteosarcoma cell lines by qRT-PCR and western blotting assays. (F, G) CCK8 and colony formation assays demonstrating the proliferation capacity ability after knocking down SFXN4 and SQOR in osteosarcoma cell lines. ∗ p < 0.05, ∗∗ p < 0.01.

Article Snippet: Human osteosarcoma cell lines (HOS, 143B) and human normal osteoblast cell lines (hFOB1.19) were purchased from Procell Life Science and Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot